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Comprehensive Assessment of Functional Changes in the Organism of Healthy Rats in External and Internal Use of Silicone Low-Mineralized Mineral Water
Sergey Gushcha,
Boris Nasibullin,
Alexander Plakida,
Irina Trubka,
Veronika Volyanskaya,
Nikolay Kalinichenko,
Irina Balashova
Issue:
Volume 4, Issue 1, February 2018
Pages:
1-5
Received:
31 October 2017
Accepted:
28 December 2017
Published:
19 January 2018
Abstract: Therapeutic mineral waters (MW) are nonspecific natural modulators; they correct the state of numerous pathogenetic links in the formation of the pathological process in the organism, which allows obtaining long-term therapeutic effects (especially at the initial stages of pathology development). Positive effect from the use of MW is due to their physical and chemical composition: the presence of macro- and microelements and biologically active compounds (sodium, potassium, magnesium, silicon, boron, iron, hydrogen sulfide). MW can be dangerous, because, acting as stress factors of low intensity; they exhaust (with long-term use) adaptive-compensatory mechanisms of the organism and promote the development of disoriginal states. The purpose of the work was to determine the safety and availability of biological activity of thermal silicon low-mineralized water on the state of the organism of healthy white rats at its internal and external application. The influence of MW on the functional state of the nervous system, kidneys, liver and structural and functional organization of the internal organs of white rats was investigated due to the application of physiological, biochemical, histological and statistical methods of research. The experiment was conducted on white rats of the Wistar line, divided into three groups. I group was control, comparative. Animals of II group received silicon MW internally, and animals of group III in the form of external procedures. It was established that the external application of MW causes a more significant calming effect on the functional state of the central nervous system. The emotional state of rats of the third group contrary - activated. The use of MW in animals of group II causes significant stimulation of urinary and excretory renal function. In both groups, the biliary function of the liver is intensified (reducing the total bilirubin content of the blood due to its fractions - direct and indirect bilirubin) and reduces the activity of oxidative-reducing enzymes in the tissues of the stomach, liver and kidneys. Consequently, the use of silicon MW in animals of the II and III groups does not cause harmful or toxic phenomena. The obtained histological results indicate that the animal's organism is in a state of reduced energy supply, that is, it can be assumed that the MW forms the state of adaptation to the damaging factors. In this case, the external application of MW is not less determined than its traditional internal use.
Abstract: Therapeutic mineral waters (MW) are nonspecific natural modulators; they correct the state of numerous pathogenetic links in the formation of the pathological process in the organism, which allows obtaining long-term therapeutic effects (especially at the initial stages of pathology development). Positive effect from the use of MW is due to their p...
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Albumin and Serum Vitamin A Status of Malnourished Children
Egbe Edmund Richard,
Eworo Raymond Ekong
Issue:
Volume 4, Issue 1, February 2018
Pages:
6-11
Received:
26 November 2017
Accepted:
14 December 2017
Published:
23 March 2018
Abstract: Malnutrition remains a major health problem in the third world countries, and is a key factor to the high death rate among children below five years old. This study aimed to determine serum vitamin A, mid-upper arm circumference (MUAC), and albumin levels of malnourished children and their well-fed counterparts. A total of one hundred (100) children comprising of fifty (50) malnourished children under the age of five years and fifty (50) well-fed age-matched children were recruited into this study. Mid upper arm circumference (MUAC) measurement was used to categorize the children into malnourished and the well-fed groups. The malnourished were further classified into kwashiorkor and marasmic cohorts. Serum vitamin A and albumin levels were determined by colorimetric methods. Data were analyzed using SPSS version 20.0 statistical package, differences between and variations among groups were determined by Student’s t-test and ANOVA respectively while the association between variables by Pearson’s correlation. Differences were considered statistically significant at p≤0.05. Serum vitamin A, MUAC and albumin levels of the well-fed group were significantly higher (p<0.05) than those of the malnourished. Comparing the well-fed, the kwashiorkor and the marasmic groups, the mean serum vitamin A, MUAC and albumin of the well-fed were significantly higher (p=0.001) than those of the kwashiorkor and marasmic groups. Mean albumin level of the kwashiorkor was significantly lower (p<0.05) than that of marasmic group. Mean MUAC of the kwashiorkor subjects was significantly higher (p<0.05) than that of the marasmic subjects. Serum vitamin A was not significantly different (p=0.724) between the kwashiorkor and the marasmic groups. In the marasmic subjects mean serum vitamin A correlates negatively with albumin (r= -0.517, p=0.011). In the kwashiorkor subjects, serum vitamin A correlates negatively with albumin (r=-0.080, p=0.690). In the well-fed children, serum vitamin A correlates positively with albumin (r=0.340, p=0.016). Conclusion: malnourished children with kwashiorkor and marasmus have lower albumin, serum vitamin A and MUAC compared with the well-fed children.
Abstract: Malnutrition remains a major health problem in the third world countries, and is a key factor to the high death rate among children below five years old. This study aimed to determine serum vitamin A, mid-upper arm circumference (MUAC), and albumin levels of malnourished children and their well-fed counterparts. A total of one hundred (100) childre...
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Assessment of Bone Health After Treatment with the Consciousness Energy Healing Treated Vitamin D3 in Human Bone Osteosarcoma Cells (MG-63)
Carola Marina Sand,
Mahendra Kumar Trivedi,
Alice Branton,
Dahryn Trivedi,
Gopal Nayak,
Sambhu Charan Mondal,
Snehasis Jana
Issue:
Volume 4, Issue 1, February 2018
Pages:
12-19
Received:
7 December 2017
Accepted:
22 December 2017
Published:
20 April 2018
Abstract: Osteoporosis is associated with increased mortality and significant economic and health burden now-a-days. The study was aimed to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. The test items, were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Carola Marina Sand and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Various parameters were performed for the assessment of bone health such as ALP, collagen, and bone mineralization in human bone osteosarcoma cells (MG-63). The cell viability (MTT) data showed that the test samples were found as safe in the tested concentrations. The level of ALP was significantly increased by 420.64%, 311.08%, and 532.17% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 1µg/mL compared to the UT-DMEM + UT-Test item group. Further, the ALP level was significantly elevated by 213.69%, 135.46%, and 42.76% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 10µg/mL compared to the untreated. Collagen was significantly increased by 228.22%, 185.40%, and 256.69% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 0.1µg/mL compared to the untreated. Further, the collagen level was significantly increased by 88.61% and 130.65% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item, respectively at 1µg/mL compared to the untreated. Besides, the percent of bone mineralization was distinctly increased by 118.35%, 266.94%, and 118.25% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50µg/mL compared to the untreated. The percent of bone mineralization was distinctly increased by 88.29%, 288.75%, and 86.25% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 100µg/mL compared to the untreated. Overall, the Biofield Energy Treated vitamin D3 was tremendously improved the bone health parameters and it could be a powerful alternative nutraceutical supplement to combat vitamin D3 deficiency and fight against various bone-related disorders including osteoporosis, low bone density, osteogenesis imperfecta, Paget’s disease, rickets, osteomalacia, deformed bones, chondrodystrophia fetalis, autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.
Abstract: Osteoporosis is associated with increased mortality and significant economic and health burden now-a-days. The study was aimed to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. The test items, were divided into two parts. One part of each sample received the Consciousness Energy Healing Treat...
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Biofield Energy Enriched Vitamin D3: A New Horizons for Development of Bone Health Using MG-63 Cells
Maire Anne Mayne,
Mahendra Kumar Trivedi,
Alice Branton,
Dahryn Trivedi,
Gopal Nayak,
Mayank Gangwar,
Snehasis Jana
Issue:
Volume 4, Issue 1, February 2018
Pages:
20-28
Received:
6 February 2018
Accepted:
12 February 2018
Published:
20 April 2018
Abstract: The current study investigates the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters in vitro using MG-63 cells such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Maire Anne Mayne and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability was more than 78% with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 141.9% and 25% at 50 and 100 µg/mL, respectively in the UT-DMEM+BT-TI group as compared with the untreated group. In addition, 144.6% (100 µg/mL) and 120.6% (1 µg/mL) increase in ALP activity in BT-DMEM+UT-TI and BT-DMEM+BT-TI group, respectively as compared with the untreated group. The level of collagen was significantly increased by 58.7% and 72.6% at 1 and 10 µg/mL, respectively in the UT-DMEM+BT-TI group, while 39.7% and 101.6% at 1 and 10 µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 117.5%, 163.5%, and 169.4% at 0.1, 1, and 10 µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 110.2%, 116.8%, and 81% at 10, 50, and 100 µg/mL, respectively in UT-DMEM+BT-TI group, while 148.8%, 180.1%, and 129.4% at 10, 50, and 100 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 142%, 181.1%, and 113% at 10, 50, and 100 µg/mL, respectively as compared with the untreated group. The experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might be vital in maintaining the various clinical safety and quality of life by assisting them in maintaining optimal vitamin D levels. It regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.
Abstract: The current study investigates the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters in vitro using MG-63 cells such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples...
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