Volume 6, Issue 5, October 2020, Page: 90-99
Sero-diagnosis of Chlamydia trachomatis and Molecular Detection of Genital Oncogenic Human Papilloma Virus Among Cameroonian Women
Bernard Wabo, Department of Medical Laboratory Science, Faculty of Health Sciences, University of Buea, Buea, Cameroon
Dickson Shey Nsagha, Department of Public Health and Hygiene, Faculty of Health Sciences, University of Buea, Buea, Cameroon
Théophile Njamen Nana, Department of Obstetrics and Gynaecology, Faculty of Health Sciences, University of Buea, Buea, Cameroon
Clement Jules Nguedia Assob, Department of Medical Laboratory Science, Faculty of Health Sciences, University of Buea, Buea, Cameroon
Received: Aug. 19, 2020;       Accepted: Sep. 2, 2020;       Published: Sep. 16, 2020
DOI: 10.11648/j.ejcbs.20200605.14      View  73      Downloads  33
Abstract
Cervical cancer is a preventable public health concern ranking second among women’s cancer in Cameroon. Human papilloma virus (HPV) is the main causative agent with Chlamydia trachomatis being suggested as the co-factor. Our objective was to characterize high risk (hr) HPV types and to detect Chlamydia trachomatis antibodies among Cameroonian women with and without cervical cancer. Methods: This unmatched case-control study enrolled 100 cases with cervical cancer and 200 controls with normal cytology aged 25- 65 years in four reference hospitals in Douala and Yaoundé (Cameroon). Consented participants filled a structured questionnaire and data on socio-demographic characteristics collected. Chlamydia trachomatis antibodies were detected by the Enzyme Linked Immuno-Sorbent Assay technique (ELISA) and hr HPV- DNA by PCR technique. Descriptive statistics was conducted to provide frequencies and percentages and the logistic regression analysis to assess the association between categorical data. p < 0.05 was considered significant. Results: Our data showed 39 (39.0%) cases aged 39-52 years compared to 96 (48.0%) controls aged 25-38 years (p=0.001). We found 82 (82.0%) cases compared to 131 (65.5%) controls with hr HPV infections. HPV 16 was most prevalent being found in 29 (29.0%) cases compared to 69 (34.5%) controls. Chlamydia trachomatis IgG / hr HPV co-infections were detected in 20 (20.0%) cases compared to 33 (16.5%) controls but with no significant association with cervical cancer (aOR=1.87; 95%CI: 0.58-5.97; p=0.293). Chlamydia trachomatis IgM (aOR=3.50; 95%CI: 1.16-10.49; p=0.025) was significantly associated with cervical cancer. Conclusion: Hr HPV- DNA was high in cases than in controls. Chlamydia trachomatis single infection and Chlamydia trachomatis/hr HPV co-infections were not significantly associated to precancerous lesions thus, necessitating further investigations.
Keywords
Precancerous Lesions, Human papilloma virus, Chlamydia trachomatis, Co-infection, Case-control Study
To cite this article
Bernard Wabo, Dickson Shey Nsagha, Théophile Njamen Nana, Clement Jules Nguedia Assob, Sero-diagnosis of Chlamydia trachomatis and Molecular Detection of Genital Oncogenic Human Papilloma Virus Among Cameroonian Women, European Journal of Clinical and Biomedical Sciences. Vol. 6, No. 5, 2020, pp. 90-99. doi: 10.11648/j.ejcbs.20200605.14
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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