Volume 6, Issue 5, October 2020, Page: 78-83
Clinical and Microbiological Epidemiology of Otomycosis in the Centre Region of Cameroon
Ekpo Alfred Itor, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
Michel Noubom, Department of Biological Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon
Claude Nangwat, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
Dougue Aude Ngueguim, Department of Biochemistry, University of Yaoundé 1, Yaoundé, Cameroon
Cyrille Levis Kountchou, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
Ngouana Kammalac Thierry, Biomedical Research Unit, Sion Laboratory, Yaounde, Cameroon
Dzoyem Jean Paul, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
Tume Christopher Bonglavnyuy, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
Received: Aug. 13, 2020;       Accepted: Aug. 24, 2020;       Published: Sep. 7, 2020
DOI: 10.11648/j.ejcbs.20200605.12      View  88      Downloads  65
Otomycosis is a superficial, sub-acute or chronic infection of the external auditory canal, characterized by pruritis, inflammation, pain and itching commonly seen in tropical and subtropical regions of the world. Various host and environmental factors can predispose a person to otomycosis. However, a clinical presentation along with otoscopic observations of the patients shows fungal and bacterial infections. Proper identification of causative agents is necessary in order to prevent recurrences and complications such as hearing lost. The aim of our study was to determine the fungi and bacteria pathogens causing otomycosis and to derive association of risk factors with otomycosis of the clinically diagnosed patients. A descriptive cross-sectional study was conducted in the otorhinolaryngology department at the University Teaching hospital and the Central hospital over a period of one year. A total of 250 clinically diagnosed patients of otomycosis of age above one year were included in the study. We evaluated age and sex distribution, predisposing factors and complaints of the clinically diagnosed patients for otomycosis. All samples collected were transported and evaluated by both direct microscopic examination and culture method for bacteria and fungi examination, which were identified by standard procedures. Among 250 samples, 46.22% yielded fungal growth, 21.33% grew bacteria only and 32.44% showed mixed growth of fungi and bacteria. Major fungal isolates were Aspergillus (n=121) including 75 isolates of Aspergillus section Nigri, 20 isolates of Aspergillus section Flavi, 13 isolates of Aspergillus section Fumigati, 8 isolates of Aspergillus section Nudilante and 5 isolates of Aspergillus section Terrei. 48 isolates were identified as Candida species. Major bacterial isolates were Staphylococcus aureus (n=45) followed by Pseudomonas species (n=26), Klebsiella species (n=21), Escherichia coli (n=7) and Proteus species (n=3). This study highlights the highest isolation of Aspergillus section Nigri in cases of clinically diagnosed otomycosis patients at the two reference hospital in Yaoundé, Cameroon with high prevalence seen in patients using antibiotic eardrops as a mean of treatment from pains and itching.
Otomycosis, Aspergillus Species, Candida Species, Bacterial Pathogens, Predisposing Factor
To cite this article
Ekpo Alfred Itor, Michel Noubom, Claude Nangwat, Dougue Aude Ngueguim, Cyrille Levis Kountchou, Ngouana Kammalac Thierry, Dzoyem Jean Paul, Tume Christopher Bonglavnyuy, Clinical and Microbiological Epidemiology of Otomycosis in the Centre Region of Cameroon, European Journal of Clinical and Biomedical Sciences. Vol. 6, No. 5, 2020, pp. 78-83. doi: 10.11648/j.ejcbs.20200605.12
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Carney, A. S. Otitis externa and otomycosis. In: Gleeson MJj Jones NS, Clarke R, et al. (eds). Scott-Brown’s Otolaryngology, Head and Neck Surgery, vol 3, 7th edn. London: Hodder Arnold Publishers; 2008, 3351-7.
Jadhav, V. J., Pal, M and Mishra, G. S. Etiological significance of Candida albicans in otitis externa. Mycopathologia, 2003, 156: 313-315.
Araiza, J., Canseco, P and Bonifaz, A. Otomycosis: clinical and mycological study of 97 cases. Review of Laryngology Otol Rhinol. 2006, 127: 251–254.
Pradhan, B., Ratna Tuladhar, N and Man Amatya, R. Prevalence of otomycosis in outpatient department of otolaryngology in Tribhuvan University Teaching Hospital, Kathmandu, Nepal. Annals of Otology, Rhinology and Laryngology, 2003, 112: 384–387.
Pontes, Z. B., Silva, A. D, Lima Ede, O., Guerra, M. H., Oliveira, N. M., Carvalho, M. F and Guerra, F. S. Otomycosis: a retrospective study. Brazilian Journal of Otorhinolaryngology, 2009; 75: 367–370.
Kurnatowski, P and Filipiak, A. Otomycosis: prevalence, clinical symptoms, therapeutic procedure. Mycoses, 2001; 44: 472-479.
Paulose, K. O., Khalifa, A. L. S., Shenoy, S. H and Sharma, R. K. Mycotic infection of ear (Otomycosis): Aprospective study. Journal of Laryngology and Otology, 1989; 103: 30-35.
Pakshir, K; Sabayan, B; Javan, H; Karamifar, K. Mycoflora of human external auditory canal in Shiraz, southern Iran. Iran Red Crescent Med J, 2008; 10: 27–29.
Gharaghani, M., Seifi, Z and Mahmoudabadi, A. Z. Otomycosis in Iran: A Review of Mycopathology, 2015; 179: 415–424.
Cheraghsahar, S., Kazemi, S, Birjandi, M., Yarahmadi, M., Mahmoudi, S., Mohammadi, R and Sepahvand, A. Otomycosis in Western Iran: clinical and mycological aspects. Arch Clin Infect Dis; 2017; 12: e57287.
Viswanatha, B., Sumatha, D and Vijayashree, M. S. Otomycosis in immunocompetent and immunocompromised patients: comparative study and literature review. Ear, Nose and Throat Journal, 2012; 91: 114–121.
Ozcan, K. M., Ozcan M., Karaarslan, A and Karaarslan, F. Otomycosis in Turkey: predisposing factors, aetiology and therapy. Journal of Otolaryngology, 2003; 117: 39-42.
Kujundzic, M, Braut T, Manestar D, Cattunar, A., Malvic, G., Vukelic, J., Puselja, Z and Linsak, D. T. Water related otitis externa. Collegium Antropologicum, 2012; 36: 893-897.
Satish, H. S., Viswanatha, B and Manjuladevi, M. A clinical study of otomycosis: Journal of Dental and Medical Sciences, 2013; 5: 57-62.
Chander, J., Maini, S., Subrahmanyan, S andHanda, A. Otomycosis: A clinico-mycological study and efficacy of mercurochrome in its treatment. Mycopathologia. 1996; 135: 9-12.
Collee, J. G., Miles, R. S., and Watt, B. Tests for identification of bacteria, in Mackie and McCartney’s Practical Medical Microbiology,. Collee J. G, Fraser A. G, Marmion B. P., and Simmons A., Editors, Churchill Livingstone, New York, NY, USA, 1996. pp. 131–145.
Fischer, F and Cook, M. Some opportunistic fungiand yeasts and yeast-like fungi. Fund Diagn Mycol. 1998.
Gokhale, S. J., Suligavi, S. S., Baragundi, D and Manjula, R. Otomycosis: A clinico mycological study. International Journal of Medical Health Sciences, 2013; 2: 218-223.
Haja, A. N., Shaik, K. M. and Siva Subba Rao, P. Mycology of Otomycosis in a tertiary care teaching hospital. Journal of Medical Research and Dental Sciences, 2015; 3: 27-30.
Kulal B., Bhat K. S., Meundi M andKotigadde S. A microbiological study of otomycosis: Indian Journal of Microbiology Research, 2017; 4: 118-125.
Rawat S., Saxena N., Chand E. A., Garg N., Verma V and Sharma K. Cinicomycological study of otomycosis with antifungal drug susceptibility testing of Candida isolates using disk diffusion method in Kota region, Rajasthan, Indian. International Journal of Current Microbiology and Application of Sciences, 2017; 6: 3356-3366.
Kaur, R., Mittal, N., Kakkar, M., Agarwal, A. K and Mathur, M. D. Otomycosis: A clinico-mycological study. Ear, Nose and Throat Journal, 2000; 79: 606-609.
Prasad, S. C., Kotigadde, S., Shekhar, M., Thada, N. D., Prabhu P. D., Souza T andPrasad K. C. Primary Otomycosis in the Indian Subcontinent: Predisposing Factors, Microbiology, and Classification. International Journal of Microbiology, 2012; 04: 1-9.
Adoga, A. S and Idul, A. A. Otomycosis in Jos: Predisposing factors and management. Afr J Med Sci, 2014; 209-213.
Fasunla, J., Ibekwe, T and Onakoya, P. Otomycosis in western Nigeria. Mycoses 2008, 51: 67–70.
Chapparbandi, R. B., Kazi, F. N andAli, K. Otomycosis: An Overview in Hyderabad Karnataka Region. Journal of Evolution of Medical and Dental Sciences. 2014; 3: 11213-11216.
Monalisa, P., BimochProjna P., Banojini P., Sanghamitra, P., Susmita, K. S., Narasimham, M. V and Indrani, M. Clinicomycological Study of Otomycosis with Antifungal Susceptibility Testing Of Fungal Isolates. Journal of Dental and Medical Sciences, 2019; 18: 7-12.
Joy, M. J., Agarwal M. K and Samanth H. C. Mycological and bacteriological studies in otomycosis. Indian Journal of Otolaryngology, 1980; 32: 72-75.
Panchal, P., Pethani., Patel, D., Rathod, S and Shah, P. Analysis of various fungal agents in clinically suspected cases of Otomycosis. Indian Journal of Basic and Applied Medical Research, 2013; 2: 865-869.
Prasanna, V., Hemlata Katiyar, V. M. and Kannan, I. Study of etiological factors, mycological profile and treatment outcome of otomycosis. International Journal of Medical Research Review, 2014; 2: 355-360.
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