Volume 6, Issue 1, February 2020, Page: 9-13
Phenotypic Characteristics of Klebsiella pneumoniae Extended Spectrum β-Lactamases Producers Isolated in Hospitals in the Littoral Region, Cameroon
Jean Teghonong, Department of Biological Sciences, Distant Production House University, Uvira, Democratic Republic of Congo; Diagmed Laboratory, Douala, Cameroon
Cecile Okalla Ebongue, Department of Biological Sciences, Douala University, Douala, Cameroon
Callixte Yadufashije, Ruhengeri-Institute of Applied Sciences, Musanze, Rwanda
Loick Kojom, Department of Animal Biology and Physiology, Douala University, Douala, Cameroon
Dieudonne Adiogo, Department of Biological Sciences, Douala University, Douala, Cameroon
Received: Jan. 8, 2020;       Accepted: Jan. 27, 2020;       Published: Feb. 11, 2020
DOI: 10.11648/j.ejcbs.20200601.13      View  207      Downloads  84
Abstract
Background: the resistance of enterobacteriaceae to antibiotics is experiencing a worrying worldwide development with an increasing impact of β-lactamases. Klebsiella pneumoniae is one of the bacteria responsible for nosocomial infections. Hence the need to look for these enzymes in the hospitals of the Littoral region, Cameroon. Method: cross-sectional and descriptive study during 2016 and early 2017, isolates of intermediate sensitivity or resistant to third generation cephalosporins (C3G) collected in the laboratories of the Littoral referral hospitals and in Diagmed laboratory. Confirmation of the strains made by the API 20E™ gallery, the study of antibiotic sensitivity by the method of diffusion of discs on agar (Mueller Hinton). The double synergy test for the search of extended spectrum β-lactamases (ESBL), and confirmation by the Eurobio ™ kit (France). Results: a total of 412 strains of klebsiellasp (species) were isolated and 122 Klebsiella pneumoniae included. The majority age group is that ≤1 year with 26.0%, the germs were isolated in the urine at 54.6% and in neonatology and pediatrics at 35.8%. Klebsiella pneumoniae pneumoniae identified at 82.4%. The production of BLSE was 86.9%, against that of AmpC at 3.30%. The sensitivity to imipenem and amikacin was 96.2% and 98.1%, respectively. Conclusion: the study shows a high frequency of ESBL and a low presence of ampC in the Littoral region, Cameroon; practitioners must make a rational prescription from a correctly performed antibiogram.
Keywords
Klebsiella pneumoniae, Resistance, β-Lactamase
To cite this article
Jean Teghonong, Cecile Okalla Ebongue, Callixte Yadufashije, Loick Kojom, Dieudonne Adiogo, Phenotypic Characteristics of Klebsiella pneumoniae Extended Spectrum β-Lactamases Producers Isolated in Hospitals in the Littoral Region, Cameroon, European Journal of Clinical and Biomedical Sciences. Vol. 6, No. 1, 2020, pp. 9-13. doi: 10.11648/j.ejcbs.20200601.13
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Freney J, Leclerq R, Riegel P. Précis de Bactériologie clinique. 2è édition. Paris: Editions ESKA; 2007. p. 1012.
[2]
Giske CG, Monnet DL, Cars O, Carmeli Y. Clinical and Economic Impact of Common Multidrug-Resistant Gram-Negative Bacilli. Antimicrobial Agents and Chemotherapy. 2008; 52 (3): 813–21.
[3]
Okalla-Ebongue C, Dongmo-Tsiazok M, Nda-Mefo'o JP, Ngaba GP, Beyiha G, Adiogo D. Evolution de la résistance aux antibiotiques des entérobactéries isolées à l'Hôpital Général de Douala de 2005 à 2012. Pamj. 2015; 20: 227.4770.
[4]
El bouamri MC, Arsalane L, Kamouni Y, Berraha M, Zouhair S. Évolution récente du profil épidémiologique des entérobactéries uropathogènes productrices de β-lactamases à spectre élargi à Marrakech, Maroc. Elsevier Masson SAS. 2014; 24 (7): 451-455.
[5]
Camara M, Diop Ndiaye H, Ba-Diallo A, Karam F, Mbow M, Faye A et al. Epidémiologie des souches de Klebsiella pneumoniae productrices de β-lactamases à spectre élargi dans un hôpital universitaire au Sénégal, 2011. Rev. CAMES SANTE. 2013; 1 (2): 133–135.
[6]
Djuikoue IC, Woerther PL, Toukam M, Burdet C, Ruppé E, Gonsu KH et al. Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon. J Infect Dev Ctries. 2016 Oct 31; 10 (10): 1135-1139.
[7]
Gangoué-Piéboji J, Branka B, Koulla-Shiro S, Randegger C, Adiogo D, Ngassam P et al. Extended-Spectrum-β-Lactamase-Producing Enterobacteriaceae in Yaounde, Cameroon. J Clin Microbiology. 2005; 34 (7): 3273-3277.
[8]
Magoué -Lonchel C, Meex C, Gangoué-Piéboji J, Boreux R, Okomo- Assoumou MC et al. Proportion of extended-spectrum ß-lactamase-producing Enterobacteriaceaein community setting in Ngaoundere, Cameroon. BMC Infect Dis. 2012; 12: 53.
[9]
Okalla-Ebongue C, Nkodo MR, Nda-Mefo JP, Temfack E, Mengue ER, Adiogo D. Phenotypic Detection of Extended Spectrum β-Lactamase and AmpC producing EnterobacteriaceaeIsolated in A General Hospital. J Microbiol Infect Dis. 2018; 8 (3): 113-119.
[10]
Chafa-Betbeui A, Gonsu-Kamga H, Toukam M, Mbakop CD, Enjema-Lyonga E, Bilong S et al. Phenotypic Detection of Extended Spectrum Beta-Lactamase and Carbapenemases Produced by Klebsiellaspp Isolated from Three Referrals Hospitals in Yaounde, Cameroon. Br Microbiology Res J. 2015; 9 (1): 1-9.
[11]
Flokas ME, Karanika S, Alevikazakos M, Mylonakis E. Prevalence of ESBL-Producing Enterobacteriaceae in Pediatric Bloodstream Infections: A Systematic Review and Meta-Analysis. PLOS ONE. 2017 Jan 31; 12 (1): e0171216. doi: 10.1371/journal.pone.0171216.
[12]
Farah A, Boutefnouchef N, Dekhil M, Bouzerna N. Klebsiella pneumoniae productrice de BLSE isolées dans les hôpitaux de la ville d’Annaba, Algérie. Scientific Study & Research. 2007; VIII (2): 1582-540X.
[13]
Feizabadi MM, Mahamadi-Yeganeh S, Mirsalehian A, Mirafshar SM, Mahboobi M, Nili F, et al. Genetic characterization of ESBL producing strains of Klebsiella pneumoniaefromTehran hospitals. J Infect Dev Ctries. 2010; 4 (10): 609-615.
[14]
Dedeic-Ljubovic A, Hukic M, Pfeifer Y, Witte W, Padilla E, López-Ramis I, Albertí S. Emergence of CTX-M-15 extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates in Bosnia and Herzegovina. Clin Microbiol Infect. 2010; 16 (2): 152-6.
[15]
Elhani D, Bakir L, Mahjoub A. Changement de l’épidémiologie de Klebsiella pneumoniae productrice de β-lactamases à spectre élargi. J Pat Bio. 2011; 69 (5): 523-9.
[16]
Hailaji NSM, Ould-Salem ML, Ghaber SM. La sensibilité aux antibiotiques des bactéries Uropathogènes dans la ville de Nouakchott-Mauritanie. J. Purol. 2016; 26 (6): 346-352.
Browse journals by subject