Escherichia coli (E. coli) is a common bacterium that can cause significant diseases in both humans and animals. The growing threat of antimicrobial resistance (AMR) poses serious risks to public health and food safety, contributing to treatment failures, increased morbidity, and rising healthcare costs. This study, conducted in Chelenko town, Ethiopia, aimed to isolate E. coli and assess its antimicrobial resistance along the beef value chain. The cross-sectional study, carried out from March to September 2022, sought to isolate and identify E. coli in beef samples and evaluate the hygienic practices in abattoirs and butcher shops within Chelenko town, East Hararghe zone, Oromia State, Ethiopia. A total of 384 samples were collected, including 78 beef meat samples, 36 feces samples, 36 water samples, and 234 swab samples from abattoir and butcher staff. Additionally, semi-structured interviews and site observations were used to assess hygienic practices. E. coli was detected in 33 (8.6%) of the total samples, with 16 (7.41%) positive samples originating from abattoirs and 17 (10.12%) from butcher shops. In abattoirs, E. coli was isolated from 2.8% of meat, 2.8% of hand swabs, 2.8% of knife swabs, 19.44% of feces, and 2.8% of water samples. In butcher shops, it was detected in 21.4% of meat, 2.4% of hand swabs, 2.4% of knife swabs, and 11.9% of feces samples. Antimicrobial susceptibility testing revealed high resistance levels, with 97% of isolates resistant to Enrofloxacin, 78.8% to Oxytetracycline and Streptomycin, 72.8% to Tetracycline, and 63.6% to Gentamycin. All E. coli isolates showed complete resistance to Amoxicillin. The study also highlighted poor hygiene practices in both abattoirs and butcher shops, emphasizing the urgent need for improved food safety training and infrastructure to ensure better hygiene in the beef supply chain.
| Published in | European Journal of Clinical and Biomedical Sciences (Volume 10, Issue 4) |
| DOI | 10.11648/j.ejcbs.20241004.12 |
| Page(s) | 57-71 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Abattoir, Beef Meat, Butcheries, Chelenko, Escherichia Coli
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APA Style
Mekonnen, A. S., Mumed, B. A., Dawed, A. (2024). Food Safety, Isolation and Antibiogram Escherichia Coli Along Beef Value Chain in Chelenko Town, Eastern Ethiopia. European Journal of Clinical and Biomedical Sciences, 10(4), 57-71. https://doi.org/10.11648/j.ejcbs.20241004.12
ACS Style
Mekonnen, A. S.; Mumed, B. A.; Dawed, A. Food Safety, Isolation and Antibiogram Escherichia Coli Along Beef Value Chain in Chelenko Town, Eastern Ethiopia. Eur. J. Clin. Biomed. Sci. 2024, 10(4), 57-71. doi: 10.11648/j.ejcbs.20241004.12
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Download@article{10.11648/j.ejcbs.20241004.12,
author = {Abnet Shewafera Mekonnen and Bayan Ahmed Mumed and Abrahim Dawed},
title = {Food Safety, Isolation and Antibiogram Escherichia Coli Along Beef Value Chain in Chelenko Town, Eastern Ethiopia
},
journal = {European Journal of Clinical and Biomedical Sciences},
volume = {10},
number = {4},
pages = {57-71},
doi = {10.11648/j.ejcbs.20241004.12},
url = {https://doi.org/10.11648/j.ejcbs.20241004.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20241004.12},
abstract = {Escherichia coli (E. coli) is a common bacterium that can cause significant diseases in both humans and animals. The growing threat of antimicrobial resistance (AMR) poses serious risks to public health and food safety, contributing to treatment failures, increased morbidity, and rising healthcare costs. This study, conducted in Chelenko town, Ethiopia, aimed to isolate E. coli and assess its antimicrobial resistance along the beef value chain. The cross-sectional study, carried out from March to September 2022, sought to isolate and identify E. coli in beef samples and evaluate the hygienic practices in abattoirs and butcher shops within Chelenko town, East Hararghe zone, Oromia State, Ethiopia. A total of 384 samples were collected, including 78 beef meat samples, 36 feces samples, 36 water samples, and 234 swab samples from abattoir and butcher staff. Additionally, semi-structured interviews and site observations were used to assess hygienic practices. E. coli was detected in 33 (8.6%) of the total samples, with 16 (7.41%) positive samples originating from abattoirs and 17 (10.12%) from butcher shops. In abattoirs, E. coli was isolated from 2.8% of meat, 2.8% of hand swabs, 2.8% of knife swabs, 19.44% of feces, and 2.8% of water samples. In butcher shops, it was detected in 21.4% of meat, 2.4% of hand swabs, 2.4% of knife swabs, and 11.9% of feces samples. Antimicrobial susceptibility testing revealed high resistance levels, with 97% of isolates resistant to Enrofloxacin, 78.8% to Oxytetracycline and Streptomycin, 72.8% to Tetracycline, and 63.6% to Gentamycin. All E. coli isolates showed complete resistance to Amoxicillin. The study also highlighted poor hygiene practices in both abattoirs and butcher shops, emphasizing the urgent need for improved food safety training and infrastructure to ensure better hygiene in the beef supply chain.
},
year = {2024}
}
TY - JOUR T1 - Food Safety, Isolation and Antibiogram Escherichia Coli Along Beef Value Chain in Chelenko Town, Eastern Ethiopia AU - Abnet Shewafera Mekonnen AU - Bayan Ahmed Mumed AU - Abrahim Dawed Y1 - 2024/11/29 PY - 2024 N1 - https://doi.org/10.11648/j.ejcbs.20241004.12 DO - 10.11648/j.ejcbs.20241004.12 T2 - European Journal of Clinical and Biomedical Sciences JF - European Journal of Clinical and Biomedical Sciences JO - European Journal of Clinical and Biomedical Sciences SP - 57 EP - 71 PB - Science Publishing Group SN - 2575-5005 UR - https://doi.org/10.11648/j.ejcbs.20241004.12 AB - Escherichia coli (E. coli) is a common bacterium that can cause significant diseases in both humans and animals. The growing threat of antimicrobial resistance (AMR) poses serious risks to public health and food safety, contributing to treatment failures, increased morbidity, and rising healthcare costs. This study, conducted in Chelenko town, Ethiopia, aimed to isolate E. coli and assess its antimicrobial resistance along the beef value chain. The cross-sectional study, carried out from March to September 2022, sought to isolate and identify E. coli in beef samples and evaluate the hygienic practices in abattoirs and butcher shops within Chelenko town, East Hararghe zone, Oromia State, Ethiopia. A total of 384 samples were collected, including 78 beef meat samples, 36 feces samples, 36 water samples, and 234 swab samples from abattoir and butcher staff. Additionally, semi-structured interviews and site observations were used to assess hygienic practices. E. coli was detected in 33 (8.6%) of the total samples, with 16 (7.41%) positive samples originating from abattoirs and 17 (10.12%) from butcher shops. In abattoirs, E. coli was isolated from 2.8% of meat, 2.8% of hand swabs, 2.8% of knife swabs, 19.44% of feces, and 2.8% of water samples. In butcher shops, it was detected in 21.4% of meat, 2.4% of hand swabs, 2.4% of knife swabs, and 11.9% of feces samples. Antimicrobial susceptibility testing revealed high resistance levels, with 97% of isolates resistant to Enrofloxacin, 78.8% to Oxytetracycline and Streptomycin, 72.8% to Tetracycline, and 63.6% to Gentamycin. All E. coli isolates showed complete resistance to Amoxicillin. The study also highlighted poor hygiene practices in both abattoirs and butcher shops, emphasizing the urgent need for improved food safety training and infrastructure to ensure better hygiene in the beef supply chain. VL - 10 IS - 4 ER -
College of Veterinary Medicine, Haramaya University, Dire Dawa, Ethiopia;Veterinary Drug and Feed Administration and Control Authority (VDFACA), Ethiopian Minster of Agriculture, Addis Ababa, Ethiopia
College of Veterinary Medicine, Haramaya University, Dire Dawa, Ethiopia
Veterinary Drug and Feed Administration and Control Authority (VDFACA), Ethiopian Minster of Agriculture, Addis Ababa, Ethiopia
