European Journal of Clinical and Biomedical Sciences

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Isolation of Selected Bacterial Pathogens from Bovine Mastitis in Selected Dairy Farms Found in Dire Dawa Town, Eastern Ethiopia

Received: 4 September 2023    Accepted: 21 September 2023    Published: 11 January 2024
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Abstract

Mastitis is a complex and costly dairy cattle disease. The main aim of this study to isolate bacterial pathogens causes bovine mastitis. A cross-sectional study was conducted from June 2022 to November 2022 to estimate the prevalence of mastitis in lactating cows, to assess the risk factors for bovine mastitis, and to isolate and identify coliform and gram-positive cocci bacteria involved in the mastitis cases from dairy farms in Dire Dawa, Eastern Ethiopia. Detection of mastitic animal was done based on physical examination of udders and CMT test. Bacterial culture and biochemical tests were employed to identify the target pathogens. A total of 366 dairy cows and 1,464 quarters were screened for mastitis. Overall prevalence of mastitis at cow and quarter levels were 24.04 and 13.5%%, respectively. Age, parity, cows udder position, history of mastitis, barn floor, milking sequences of clinically mastitic cows and leg and udder hygiene scores were found to be risk factors significantly (P<0.05) associated with mastitis. From the 191 mastitis-positive milk samples, 82.7% (158/191) were culture positive. Out of the isolates from clinical cases (n=59) and isolates from sub clinical cases (n=99), Staphylococcus aureus (22%) and E. coli (15.7%) were predominant isolate. The other bacterial isolate in order of abundance, Coagulase Negative Staphylococcus (10.5%), Streptococcus agalactiae (6.8%), Streptococcus dysgalactiae (5.8%), Staphylococcus intermedius (4.7%), Staphylococcus hyicus (4.2%), Klebsiella pneumoniae (3.1%), Micrococci spp (2%), Streptococcus uberis (1.6%), Enterobacter aerogenes (1%), and Enterococci spp (0.5%). The study showed that high parity number (OR = 19.5; p = 0.005), moderate parity (OR = 10.9; p = 0.022) and history of mastitis in preceding lactation (OR = 28.4; p = 0.001) were the major risk factors which are significantly associated with higher prevalence of S. aureus. History of mastitis in preceding lactation (OR = 3.7; p = 0.021) and very dirty (OR = 3.9; p = 0.005) udder and legs were the major risk factors which are significantly associated with higher prevalence of E. coli. Therefore, hygienic milking practice, adequate sanitation of the dairy environment, proper attention to the health of mammary glands and regular screening tests should get emphases as control strategies.

DOI 10.11648/j.ejcbs.20241001.11
Published in European Journal of Clinical and Biomedical Sciences (Volume 10, Issue 1, February 2024)
Page(s) 1-14
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Associated Risk Factor, CMT, Coliform, Isolation, Gram Positive Cocci, Mastitis, Prevalence

References
[1] O. M. Radostits, C. C. Gay, K. W. Hinchcliff and P. D. Constable. Veterinary Medicine: A Textbook of the Disease of Cattle, Horses, Sheep, Pigs and Goats. 10th ed. London: Elsevier. 2007.
[2] S. Taponen, E. Liski, A. M. Heikkila, and S. Pyorala,”Factors associated with intramammary infection in dairy cows caused by coagulase-negative staphylococci, Staphylococcus aureus, Streptococcus uberis, Streptococcus dysgalactiae, Corynebacterium bovis, or Escherichia coli”. Journal of Dairy Science, vol. 100, no. 1, pp. 493–503, 2017.
[3] A. Ababu, D. Endashaw, H. Fesseha, “Isolation and antimicrobial susceptibility profile of Escherichia coli O157: H7 from raw milk of dairy cattle in Holeta District, Central Ethiopia”. Int J Microbiol, vol. 20, no. 8. doi: 10.1155/2020/6626488. 2020.
[4] M. Pal, A. Regasa, F. Gizaw, Etiology, “Pathogenesis, Risk Factors, Diagnosis and Management of Bovine Mastitis: A Comprehensive Review,” International Journal of Animal and Veterinary Sciences, vol. 06, pp. 40-55. 2019.
[5] P. J. Quinn, B. K. Markey, F. C. Leonard, E. S. FitzPatrick, S. Fanning, and P. J. Hartigan, Veterinary Microbiology and Microbial Disease. 2nd edition. West Sussex, United Kingdom: Wiley-Blackwell. pp. 912, 2011.
[6] R. J. Erskine, ‘‘Intramuscular administration of ceftiofursodiu versus intra mammary infusion of penicillin/novobiocin for treatment of Streptococcus agalactiae mastitis in dairy cows,”J Am Vet Med Assoc, vol. 208, pp. 258–260, 2001.
[7] P. R. F. Adkins, and J. R. Middleton, ‘‘Methods for diagnosing mastitis,” Vet Clin North Am Food Anim Pract, Vol. 34, pp. 479–91 doi: 10.1016/j.cvfa.2018.07.003. 2018.
[8] P. L. Ruegg. 100-year review: mastitis detection, management, and prevention. Journal of Dairy Science, Vol. 100, no. 12, pp. 10381–10397, 2017.
[9] H. W. Jamali, M. Barkema, Jacques et al., Invited review: incidence, risk factors, and effects of clinical mastitis recurrence in dairy cows. Journal of Dairy Science, vol. 101, no. 6, pp. 4729–4746, 2018.
[10] G. Jones, and T. L. Bailey, ‘‘Understanding the basics of mastitis. Virginia Cooperative Extension Publication” No. 404-233, Virginia State University, USA, 2009.
[11] K. D. Oudessa, Bovine Mastitis: Part I. Animal Reproduction in Veterinary Medicine. doi: http://dx.doi.org/10.5772/intechopen.934832020.
[12] T. Bobbo, P. L. Ruegg, G. Stocco, E. Fiore, M. Gianesella, M. Morgante, ‘‘Associations between pathogen specific cases of subclinical mastitis and milk yield, quality, protein composition, and cheese making traits in dairy cows,” Journal of Dairy Science, Vol. 100, pp. 4868-4883, 2017.
[13] T. E. Motaung, K. R. Petrovski, I. M. Petzer, O. Thekisoe, T. J. Tsilo, “Importance of bovine mastitis in Africa,” Animal Health Research Reviews, vol. 18, no. 1, pp. 58–69, 2017.
[14] A. M. Heikkilä, E. Liski, S. Pyörälä, S. Taponen, “Pathogen-specific production losses in bovine mastitis,” J. Dairy Sci, vol. 101, pp. 9493–9504, 2018.
[15] A. J. Bradley, K. L. Leach, J. E. Breen, L. E. Green, and M. J. Green, “Survey of the incidence and aetiology of mastitis on dairy farms in England and Wales,” Veterinary Record, vol. 160, no. 8, pp. 253–258, 2007.
[16] R. N. Zadoks, J. R. Middleton, S. McDougall, J. Katholm, Y. H. Schukken, “Molecular epidemiology of mastitis pathogens of dairy cattle and comparative relevance to humans,” J. Mammary Gland Biol, vol. 164, pp. 357–372, 2011.
[17] M. Pal, Mastitis: A major production disease of dairy animals. Agriculture World, vol. 4, pp. 46-51. 2018.
[18] G. Wellenberg, P. Vander, and J. Oirschot, “Viral infections and bovine mastitis a review,” Veterinary Microbiology, vol. 88, pp. 27-45, 2002.
[19] K. R. Petroviski, M. Trajcev, and G. Buneski, “A review of the factors affecting the costs of bovine mastitis,” J. South. Afr. Vet. Ass, vol. 77, no. 5, pp. 52-60, 2006.
[20] M. Mohamed, A. S. Ehui, and Y. Assefa, “Dairy development in Ethiopia,” EPTD Discussion Paper 123, International Food Policy Research Institute, Washington, DC, USA, 2006.
[21] D. Biffa, E. Debela, and F. Beyene, “Prevalence and risk factor of mastitis in lactating dairy cow in southern Ethiopia,” International Journal of Applied Research of Veterinary medicine, vol. 3, pp. 189-198. 2005.
[22] F. J. S. Van Soest, I. M. G. A. Santman-Berents, T. J. G. M. Lam, and H. Hogeveen, “Failure and preventive costs of mastitis on Dutch dairy farms,” J. Dairy Sci, vol. 99, pp. 8365-8374, 2016.
[23] P. Kumar, A. D. Ojasvita, H. Sharma, S. Sharma, D. Mittal, V. Bhanot, A. Prakash, R. Yadav, and R. P. Diwakar, Bovine Mastitis: A Review. Middle-East Journal of Scientific Research, vol. 28, no. 6, pp. 497-507, 2020.
[24] FAO (Food and Agricultural Organization): Livestock Sector Brief. Livestock information, sector analysis and policy branch. Food and Agricultural Organization, Rome, Italy. April 1-15. 2003.
[25] D. Morin, G. Petersen, H. Whitmore, L. Hungerfold and R. Hinton, “Economic analysis of a mastitis monitoring and control program in four dairy herds,” J. Am. Vet. Med. Assoc, Vol 202, pp. 540-548, 1993.
[26] E. O. Mungube, Management and economics of dairy cow mastitis in urban and peri-urban areas of Addis Ababa milk shed, MSc. Thesis, Addis Ababa University FVM, Debrezeit, Ethiopia. 2001.
[27] E. O. Mungube, B. A. Tenhagen, F. Regassa, M. N. Kuyle, Y. Sheferaw, T. Kassa and M. P. O. Baumann, “Reduced milk production in udder quarters with subclinical mastitis and associated economic losses in crossed breed dairy cows in Ethiopia,” Trop Anim Health Prod, Vol. 37, pp. 503- 512, 2005.
[28] CSA (Central Statistical Agency), Report on livestock and livestock characteristics. The Federal Democratic Republic of Ethiopia, Private peasant holdings, Statistical bulletin 570. Addis Ababa, Ethiopia. 2020.
[29] K. Fekadu, “Survey on the prevalence of bovine mastitis and the predominant causative agents in Chaffa valley,” Proceedings of the 9th Conference of Ethiopian Veterinary Association: Addis Ababa, Ethiopia, pp. 101-111, 1995.
[30] D. Kerro, and F. Tareke, “Bovine mastitis in selected areas of Southern, Ethiopia,” Trop Anim. Health Prod, Vol. 35, no. 3, pp. 197-205, 2003.
[31] S. Girum, “Mastitis Causative Agent in Amahara regional state, Ethiopia” Veterinary Journal, Vol. 1, pp. 8-13, 2009.
[32] I. Ejeta, S. Biressaw and M. Pal, “Studies on the Prevalence of Bovine Mastitis and Its Associated Risk Factors in and Around Ambo Town Oromia Region Central Ethiopia,” International Journal of Livestock Research, Vol. 12, no. 3, pp. 15-25, 2022.
[33] H. Fesseha, M. Mathewos, S. Aliye, A. Wolde, “Study on Prevalence of Bovine Mastitis and Associated Risk Factors in Dairy Farms of Modjo Town and Suburbs, Central Oromia, Ethiopia,” Veterinary Medicine: Research and Reports, Vol. 12, pp. 271–283, 2021.
[34] M. Etifu and M. Tilahun, “Prevalence of bovine mastitis, risk factors, isolation and anti-bio gram of major pathogens in Mid Rift valley, Ethiopia,” Int. J. Livest. Prod, Vol. 10, no. 1, pp. 14-23, 2019.
[35] R. Abebe, A. Mesele, D. Yifat, S. Mishamo, F. Amene, A. Fufa, A. Adugna, W. Demisie, K. Erdachew, T. Beyene, “Prevalence, risk factors and bacterial causes of bovine mastitis in southern Ethiopia,” Ethiopian Veterinary Journal, Vol. 24, no. 1, pp. 52-68, 2020.
[36] B. Tesfaye and A. Abera, “Prevalence of mastitis and associated risk factors in Jimma town dairy farms, Western Ethiopia,” J Vet Sci Anim Husb, Vol. 6, no. 3, pp. 307, 2018.
[37] K. Jafer, D. Haimanot, J. Hawi, Z. Tilahun and K. Girma, “A Study on Bovine Mastitis, Isolation and Identification of Staphylococcus Species in Dairy Farms of Dire Dawa City, Eastern Ethiopia,” Global Veterinaria, Vol. 16, no. 3, pp. 222-230, 2016.
[38] T. Biniam, T. Rediet and A. Yonus, “Prevalence and potential risk factors of bovine mastitis in selected dairy farms of dire Dawa town, Eastern Ethiopia,” Appl. J. hygiene, Vol. 4, no. 1, pp. 06–11, 2015.
[39] G. Darsema, A survey of Bovine mastitis in different dairy farms: Dire Dawa Autonomous and East Harerge Administrative Regions. DVM Thesis: Addis Ababa University Addis Ababa, Ethiopia. 1991.
[40] DDAEPA (Dire Dawa Administration Program Adaptation to climate change), Dire Dawa administration program of adaptation to climate change. DDAEPA, Dire Dawa, Ethiopia, pp. 5-6, 2018.
[41] B. W. Bedacha and H. T. Mengistu, “Study on Prevalence of Mastitis and its Associated Risk Factors In Lactating Dairy Cows in Batu and its Environments, Ethiopia,” Global Veterinaria, Vol. 7, no. 6, pp. 632-637, 2011.
[42] M. V. Thrusfield. Sample size determination In Veterinary Epidemiology, 3rd edition. Blackwell Science, Ltd., oxford England, 2005.
[43] D. Wakeman and J. Pace, “Determining the age of cattle by their teeth,” J Am Vet Med Assoc, Vol. 121, pp. 483, 1983.
[44] D. A. Schreiner and P. L. Ruegg, “Relationship between udder and leg hygiene scores and subclinical mastitis,” J Dairy Sci, Vol. 86, pp. 346, 2003.
[45] National Mastitis Council, (NMC): Microbiological procedures for the diagnosis of udder infection: 3rded, National Mastitis Council Inc. Arlington, 2, 258 260, 2004.
[46] P. Quinn, M. Carter, B. Markey and G. Carter, “Clinical Veterinary Microbiology, Harcourt Publishers, Virginia, USA,” 2002.
[47] K. Yohannes and B. Alemu, “Prevalence of Bovine Mastitis in lactating Cows and Associated risk factors in and around Wolayta Soddo, Southern Ethiopia,” Int. J. Adv. Res. Biol. Sci, Vol. 5, no. 12, pp. 60-69, 2018.
[48] E. Zerfu, H. Abebe, B. Mengiste, H. Shafi, A. Jibril, A. Mezgebu, H. Mohammed, “Prevalence and Identification of Predominant Causative Agents of Bovine Mastitis in Boke Woreda of Western Hararghe Zone, Oromia Regional State, Ethiopia,” J Microbiol Biotechnol, Vol. 10, no. 3, 2021.
[49] B. Demelash, D. Etana and B. Fekadu, “Prevalence and risk factors of mastitis in lactating dairy cow in Southern Ethiopia,” Journal of Applied Research on Veterinary Medicine, Vol. 3, pp. 189-198, 2005.
[50] S. Girma, A. Mammo, K. Bogele, T. Sori, F. Tadesse and T. Jibat, “Study on prevalence of bovine mastitis and its major causative agents in West Harerghe zone, Doba district, Ethiopia,” J. Vet. Med. Anim. Hlth, Vol. 4, no. 8, pp. 116-123, 2012.
[51] N. Belay, N. Mohammed and W. Seyoum, “Bovine Mastitis: Prevalence, Risk Factors, and Bacterial Pathogens Isolated in Lactating Cows in Gamo Zone, Southern Ethiopia,” Veterinary Medicine: Research and Reports, Vol. 13, pp. 9–19, 2022.
[52] A. Kasech, T. Alebachew and A. Alemu, “Study on prevalence of Bovine mastitis in Tullo District of West Hararghe, Ethiopia: a cross sectional study,” Adv Bio Res, Vol. 10, pp. 147–153, 2016.
[53] B. Tesfaye, “Bovine mastitis prevalence, isolation of bacterial species involved and its antimicrobial susceptibility test around Debrezeit, Ethiopia: a cross-sectional study.” J Vet Sci Technol, Vol. 7, pp. 396, 2016.
[54] F. Abraham and M. Zeleke, “Prevalence of Bovine Clinical mastitis and farmer’s awareness in and around Wolaita Sodo, Southern Ethiopia,” J Adv Dairy Res, Vol. 5, pp. 184, 2017.
[55] A. M. Getaneh and E. Z. Gebremedhin, “Meta-analysis of the prevalence of mastitis and associated risk factors in dairy cattle in Ethiopia,” Trop. Anim. Hlth. Prod, Vol. 49, PP. 697-705, 2017.
[56] R. Abebe, H. Hatiya, M. Abera, B. Megersa and K. Asmare, “Bovine mastitis: prevalence, risk factors and isolation of Staphylococcus aureus in dairy herds at Hawassa milk shed, South Ethiopia,” BMC Vet. Res, Vol. 12, no. 1, pp. 270, 2016.
[57] R. H. Mdegela, R. Ryoba, E. D. Karimuribo, E. J. Phiri, T. Løken, O. Reksen, E. Mtengeti, N. A. Urio, “Prevalence of clinical and subclinical mastitis and quality of milk in smallholder dairy farms in Tanzania,” J S Afr Vet Assoc, Vol. 80, pp. 163–8, 2009.
[58] M. Christine, G. C. Mbindyo, C. Gitao and M. Charles, “Prevalence, Etiology, and Risk Factors of Mastitis in Dairy Cattle in Embu and Kajiado Counties, Kenya,” Veterinary Medicine International, pp. 12, 2020.
[59] D. T. Dabele, Snr, B. M. Borena, P. Admasu, “Prevalence and risk factors of mastitis and isolation, identification and antibiogram of Staphylococcus species from Mastitis positive Zebu cows in Toke Kutaye, Cheliya, and Dendi Districts, West Shewa Zone, Oromia, Ethiopia. Infect Drug Resist, 14: 987–998. 2021.
[60] B. Amin, Y. Deneke and N. Abdela, ‘‘Bovine mastitis: prevalence, risk factors and isolation of Streptococcus species from small holder dairy farms in and around Haramaya Town, Eastern Ethiopia” Global J of Med Res, Vol. 17, no. 1, pp. 27–38, 2017.
[61] H. Seegers, C. Fouricho and F. Beaudeau, “Production effects related to mastitis and mastitis economics in dairy cattle herds,” Vet Rec, vol. 34, pp. 475–491, 2003.
[62] B. T. Lakew, T. Fayera, Y. A. Muktar, “Risk factors for bovine mastitis with the isolation and identification of Streptococcus agalactiae from farms in and around Haramaya district, Eastern Ethiopia,” Tropical Animal Health and Production, vol. 51, pp. 1507–1513, 2019.
[63] G. Kitila, B. Kebede, and M. Wakgari, “Prevalence, aetiology and risk factors of mastitis of dairy cows kept under extensive management system in West Wollega, Western Oromia Ethiopia,” Vet Med Sci, vol. 7, no. 3, pp. 1–7, 2021.
[64] A. Sefinew, T. Firaol, A. Gizat and T. Awoke, “Study on bovine mastitis and its effect on chemical composition of milk in and around Gondar Town, Ethiopia,” Journal of Veterinary Medicine and Animal Health, vol. 5, no. 8, pp. 215-221, 2013.
[65] T. Zeryehun, and G. Abera, ‘‘Prevalence and bacterial isolates of mastitis in dairy farms in selected districts of Eastern Harrarghe Zone, Eastern Ethiopia,” Hindawi J Vet Med, vol. 7, pp. 1–3, 2017.
[66] M. Bitew, A. Tafere and T. Tolosa, “Study on bovine mastitis in dairy farms of Bahir Dar and its environs,” J Anim Vet Adv, vol. 9, no. 23, pp. 2912–2917, 2010.
[67] A. Kumbe, B. Bekele, B. Hussien, A. Onate, and D. Teshome, “Study on Bovine mastitis under different management in pastoral and agro-pastoral areas of Borana Zone, Southern Ethiopia,” J Vet Sci Res, vol. 5, no. 1, pp. 000192, 2020.
[68] O. Kocak, “Influence of mastitis on milk yields in Holstein Cows,” Acta Vet Brno, vol. 75, pp. 507–13, 2006.
[69] T. F. Demissie, H. T. Menghistu and M. A. Mitiku, “Prevalence of mastitis and identification of its bacterial causative agents in small holder dairy farms in and around Wukro of Tigray region, Ethiopia,” Int. J. Adv. Res. Biol. Sci, vol. 5, no. 11, pp. 10-22, 2018.
[70] D. Belina, A. H. Muktar, N. Tamerat, et al, “Prevalence, isolation of bacteria and risk factors of mastitis of dairy cattle in selected zones of Oromia regional states, Ethiopia,” Glob J Med Res, vol. 16, no. 1, pp. 39–42, 2016.
[71] T. Firaol, Yitagele T, Nateneal T, Endrias Z. Prevalence of coagulase positive staphylococcus in mastitis infection in dairy cattle in and around Haramaya, Ethiopian: Veterinary Journal, vol 19, no 2, pp. 41-53, 2015.
[72] M. M. Awale, G. B. Dudhatra, K. Avinash, B. N. Chauhan and D. R. Kamani, ‘‘Bovine mastitis: a threat to economy,” Sci Rep, vol. 1, pp. 295, 2012.
[73] M. Yenew, and H. Addis, ‘‘Study on the prevalence and associated risk factors of bovine mastitis in and around Dessie town, South Wollo, northeastern Ethiopia,” Biomed Nurs, vol. 6, no. 3, pp. 59–76, 2020.
[74] N. Sharma, N. K. Singh, O. P. Singh, V. Pandey, P. K. Verma, “Oxidative stress and antioxidant status during transition period in dairy cows,” Asian-Aust J Anim Sci, vol. 24, pp. 479–84, 2011.
[75] I. Rajabi, A. Taghavi Razavizadeh, M. Azizzadeh, and B. Khoramian, ‘‘The investigation on the relationship between dairy cow hygiene scores and intramammary infections,” Iranian J. Vet. Sci. Tech, vol. 9, no. 1, pp. 12-16, 2017.
[76] D. K. Mureithi and M. N. Njuguna, “Prevalence of subclinical mastitis and associated risk factors in dairy farms in urban and peri-urban areas of Thika Sub County, Kenya,” Livestock Res. Rural Dev, vol. 28, no. 2, pp. 13, 2016.
[77] Tezera, M and Ali, E. 2021. Prevalence and associated risk factors of Bovine mastitis in dairy cows in and around Assosa town, Benishangul Gumuz Regional State, Western Ethiopia. Vet Med Sci, 7: 1280–1286.
[78] M. Abera, B. Demie, K. ragaw, F. Regassa, and A. Regassa, ‘‘Isolation and identification of Staphylococcus aureus from bovine mastitic milk and their drug resistance patterns in Adama town Ethiopia,” Journal of Veterinary Medicine and Animal Health, vol. 1, no. 2, pp. 19–23, 2013.
[79] F. Kassa, A. Ayano, M. Abera and A. Kiros, “Longitudinal study of bovine mastitis in Hawassa and Wendo Genet Small Holder Dairy Farms,” Global Journal of Science Frontier Research, Vol. 14, no. 2, pp. 33–41, 2014.
[80] J. S. Kuehn, P. J. Gorden and D. Munro, Bacterial community Profiling of milk samples as a means to understand culture negative bovine clinical mastitis,” PLoS One, vol. 8, no. 4, Article ID e61959. 2013.
[81] K. Dereje, A. Kebede, N. Abebe, Y. Tamiru, “Isolation, identification and antimicrobial susceptibility test of mastitis causing bacteria at Holeta Agricultural Research Center dairy farms,” Int J Animal Sci Technol, vol. 2, no. 1, pp. 6–13, doi: 10.11648/j.ijast.20180201.12, 2018.
[82] S. Amer, F. L. G´alvez, Y. Fukuda, et al, “Prevalence and etiology of mastitis in dairy cattle in El Oro Province, Ecuador,” Journal of Veterinary Medical Science, vol. 80, no. 6, pp. 861–868, 2018.
[83] Z. G. Endrias, B. Addisu, M. B. Bizunesh, A. K. Kebede, D. T. Nega, M. M. Lencho, and J. S. Edilu, “Isolation and Identification of Staphylococcus aureus from Milk and Milk Products, Associated Factors for Contamination, and Their Antibiogram in Holeta, Central Ethiopia,” Veterinary Medicine International, pp. 13 doi.org/10.1155/2022/6544705, 2022.
[84] U. A. Abegewi, S. N. Esemu, N. N. Roland, M. N. Lucy, “Prevalence and risk factors of coliform- associated mastitis and antibiotic resistance of coliforms from lactating dairy cows in North West Cameroon,” PLoS one, vol. 17, no. 7, pp. e0268247 doi.org/10.1371/journal.pone.0268247, 2022.
[85] C. S. Oliveira, H. Hogeveenc, A. M. Botelhod, P. V. Maiad, S. G. Coelhob, J. P. Haddada, “Cow specific risk factors for clinical mastitis in Brazilian dairy cattle,” Prev Vet Med, vol. 121, pp. 297–305, 2015.
[86] FAO (Food and Agriculture Organization), Impact of mastitis on small scale diary production systems, Animal Production and Health Working Paper. No 13. Rome. 2014.
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    Mekonnen, A. S., Yuya, H. M., Ahamed, A. S. (2024). Isolation of Selected Bacterial Pathogens from Bovine Mastitis in Selected Dairy Farms Found in Dire Dawa Town, Eastern Ethiopia. European Journal of Clinical and Biomedical Sciences, 10(1), 1-14. https://doi.org/10.11648/j.ejcbs.20241001.11

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    Mekonnen, A. S.; Yuya, H. M.; Ahamed, A. S. Isolation of Selected Bacterial Pathogens from Bovine Mastitis in Selected Dairy Farms Found in Dire Dawa Town, Eastern Ethiopia. Eur. J. Clin. Biomed. Sci. 2024, 10(1), 1-14. doi: 10.11648/j.ejcbs.20241001.11

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    Mekonnen AS, Yuya HM, Ahamed AS. Isolation of Selected Bacterial Pathogens from Bovine Mastitis in Selected Dairy Farms Found in Dire Dawa Town, Eastern Ethiopia. Eur J Clin Biomed Sci. 2024;10(1):1-14. doi: 10.11648/j.ejcbs.20241001.11

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  • @article{10.11648/j.ejcbs.20241001.11,
      author = {Abnet Shewafera Mekonnen and Hamza Mohammed Yuya and Amedine Shafi Ahamed},
      title = {Isolation of Selected Bacterial Pathogens from Bovine Mastitis in Selected Dairy Farms Found in Dire Dawa Town, Eastern Ethiopia},
      journal = {European Journal of Clinical and Biomedical Sciences},
      volume = {10},
      number = {1},
      pages = {1-14},
      doi = {10.11648/j.ejcbs.20241001.11},
      url = {https://doi.org/10.11648/j.ejcbs.20241001.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20241001.11},
      abstract = {Mastitis is a complex and costly dairy cattle disease. The main aim of this study to isolate bacterial pathogens causes bovine mastitis. A cross-sectional study was conducted from June 2022 to November 2022 to estimate the prevalence of mastitis in lactating cows, to assess the risk factors for bovine mastitis, and to isolate and identify coliform and gram-positive cocci bacteria involved in the mastitis cases from dairy farms in Dire Dawa, Eastern Ethiopia. Detection of mastitic animal was done based on physical examination of udders and CMT test. Bacterial culture and biochemical tests were employed to identify the target pathogens. A total of 366 dairy cows and 1,464 quarters were screened for mastitis. Overall prevalence of mastitis at cow and quarter levels were 24.04 and 13.5%%, respectively. Age, parity, cows udder position, history of mastitis, barn floor, milking sequences of clinically mastitic cows and leg and udder hygiene scores were found to be risk factors significantly (PStaphylococcus aureus (22%) and E. coli (15.7%) were predominant isolate. The other bacterial isolate in order of abundance, Coagulase Negative Staphylococcus (10.5%), Streptococcus agalactiae (6.8%), Streptococcus dysgalactiae (5.8%), Staphylococcus intermedius (4.7%), Staphylococcus hyicus (4.2%), Klebsiella pneumoniae (3.1%), Micrococci spp (2%), Streptococcus uberis (1.6%), Enterobacter aerogenes (1%), and Enterococci spp (0.5%). The study showed that high parity number (OR = 19.5; p = 0.005), moderate parity (OR = 10.9; p = 0.022) and history of mastitis in preceding lactation (OR = 28.4; p = 0.001) were the major risk factors which are significantly associated with higher prevalence of S. aureus. History of mastitis in preceding lactation (OR = 3.7; p = 0.021) and very dirty (OR = 3.9; p = 0.005) udder and legs were the major risk factors which are significantly associated with higher prevalence of E. coli. Therefore, hygienic milking practice, adequate sanitation of the dairy environment, proper attention to the health of mammary glands and regular screening tests should get emphases as control strategies.
    },
     year = {2024}
    }
    

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  • TY  - JOUR
    T1  - Isolation of Selected Bacterial Pathogens from Bovine Mastitis in Selected Dairy Farms Found in Dire Dawa Town, Eastern Ethiopia
    AU  - Abnet Shewafera Mekonnen
    AU  - Hamza Mohammed Yuya
    AU  - Amedine Shafi Ahamed
    Y1  - 2024/01/11
    PY  - 2024
    N1  - https://doi.org/10.11648/j.ejcbs.20241001.11
    DO  - 10.11648/j.ejcbs.20241001.11
    T2  - European Journal of Clinical and Biomedical Sciences
    JF  - European Journal of Clinical and Biomedical Sciences
    JO  - European Journal of Clinical and Biomedical Sciences
    SP  - 1
    EP  - 14
    PB  - Science Publishing Group
    SN  - 2575-5005
    UR  - https://doi.org/10.11648/j.ejcbs.20241001.11
    AB  - Mastitis is a complex and costly dairy cattle disease. The main aim of this study to isolate bacterial pathogens causes bovine mastitis. A cross-sectional study was conducted from June 2022 to November 2022 to estimate the prevalence of mastitis in lactating cows, to assess the risk factors for bovine mastitis, and to isolate and identify coliform and gram-positive cocci bacteria involved in the mastitis cases from dairy farms in Dire Dawa, Eastern Ethiopia. Detection of mastitic animal was done based on physical examination of udders and CMT test. Bacterial culture and biochemical tests were employed to identify the target pathogens. A total of 366 dairy cows and 1,464 quarters were screened for mastitis. Overall prevalence of mastitis at cow and quarter levels were 24.04 and 13.5%%, respectively. Age, parity, cows udder position, history of mastitis, barn floor, milking sequences of clinically mastitic cows and leg and udder hygiene scores were found to be risk factors significantly (PStaphylococcus aureus (22%) and E. coli (15.7%) were predominant isolate. The other bacterial isolate in order of abundance, Coagulase Negative Staphylococcus (10.5%), Streptococcus agalactiae (6.8%), Streptococcus dysgalactiae (5.8%), Staphylococcus intermedius (4.7%), Staphylococcus hyicus (4.2%), Klebsiella pneumoniae (3.1%), Micrococci spp (2%), Streptococcus uberis (1.6%), Enterobacter aerogenes (1%), and Enterococci spp (0.5%). The study showed that high parity number (OR = 19.5; p = 0.005), moderate parity (OR = 10.9; p = 0.022) and history of mastitis in preceding lactation (OR = 28.4; p = 0.001) were the major risk factors which are significantly associated with higher prevalence of S. aureus. History of mastitis in preceding lactation (OR = 3.7; p = 0.021) and very dirty (OR = 3.9; p = 0.005) udder and legs were the major risk factors which are significantly associated with higher prevalence of E. coli. Therefore, hygienic milking practice, adequate sanitation of the dairy environment, proper attention to the health of mammary glands and regular screening tests should get emphases as control strategies.
    
    VL  - 10
    IS  - 1
    ER  - 

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Author Information
  • Department of Veterinary Medicine, Haramaya University, Dire Dawa, Ethiopia

  • Department of Veterinary Medicine, Meta Woreda Veterinary Clinic, East Hararghe, Ethiopia

  • Department of Veterinary Medicine, Meta Woreda Veterinary Clinic, East Hararghe, Ethiopia

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